DMDiscoverySM

Tandem Labs offers the following service categories for metabolite screening and identification:

  • Level 1: Metabolite Screening

    Applicable to the early stage screening of compounds where the objective is to improve the PK/PD characteristics of compounds within a series that are built upon a common scaffold, e.g., identification and design mediation of compound-metabolic 'hot spots'.

  • Level 2: Metabolite Surveying

    Applicable to the later stages of discovery where the objective may be the optimization of a lead candidate compound(s) PK/PD, e.g., determination of specific sites of hydroxylation and oxidation, among others, and their relative rates.

  • Level 3: Metabolite Identification and Pathways

    Applicable to compound development (non-clinical or clinical) or candidate nomination where the objective is to comprehensively identify the full range of drug compound metabolites to assist in the design of their respective development programs, e.g., the identification of species-specific metabolites and metabolites for safety testing (MIST), among others.

Summary of Metabolite Screening and Identification Services, Strategies, and Outcomes

Technology Process
Service Category Data Collection Mass Accuracy Outcomes
LC/MS/MS Metabolite Screening and Surveying MRM Scan Unit Mass • Common metabolic alterations
• May not identify modification sites1
• Cannot identify modification types (e.g., -NH2 vs. -O)
Precursor Ion Scan
Precursor Ion Scan
Neutral Loss Scan
LC-QTOF Metabolite Surveying, ID, and Pathways LC/MS Accurate Mass • Common metabolic alterations
• May not identify modifications sites1
• Identify modifications types (e.g., -NH2 vs. -O)
LC/MS/MS
MALDI-TOF Metabolite ID and Pathways MS Accurate Mass • Common metabolic alterations
• May not identify modifications sites1
• Identify modifications types3 (e.g., -NH2 vs. -O)
MS/MS
NOTES
1 - Site of modifications contingent upon LC separation.
2 - High accurate mass MS/MS may enable determination of site modifications
3 - High energy accurate mass MS/MS enables determination of site modification.

Graphic Depiction of Accelerated Metabolite Identification Service Category as it Relates to the DruG Discovery and Development Process

Graphic Deptiction of Accelerated Metabolite Identification Service Category as it Relates to the Drub Discovery and Development Process